human osteosarcoma mg63 cell line  (ATCC)

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Representative 1 H NMR spectra of MG63 cell metabolites after being cultured on different types of PLLA. The cells were seeded on PLLA, PLLA-Cel, PLLA-Col, and PLLA-Cel-Col for 7 days. Peak assignments: 1 – lactic acid, 2 – 3-hydroxy- l -proline, 3 – glucosylgalactosylhydroxylysine, 4 – lysine, 5 – glucosylproline, 6 – pyruvic acid, 7 – succinic acid, 8 – α-ketoglutaric acid, 9 – proline, 10 – 5-hydroxylysine, 11 – oxaloacetic acid, 12 – acetyl-CoA, 13 – FADH 2 , 14 – 2-phosphoglyceric acid, 15 – cAMP, 16 – 3-phosphoglyceric acid, 17 – phosphoenolpyruvic acid, 18 – NADH. Unassigned peaks represent unknown compounds not in the HMDB database, residual solvent signals, or overlapping signals. The water signal region (4.6–5.0 ppm) was excluded from analysis to minimize interference.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Cell Culture, Solvent

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Multivariate analyses of metabolomic profiles of MG63 cells cultured on PLLA and its composites, including PLLA-Cel, PLLA-Col, and PLLA-Cel-Col, for 7 days. Each data point represents a sample, with colors indicating the scaffold type: (A) principal component analysis (PCA) scores plot and (B) partial least-squares discriminant analysis (PLS-DA) scores plot.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Cell Culture

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Metabolic pathway enrichment analysis of MG63 cells cultured on PLLA, PLLA-Cel, PLLA-Col, and PLLA-Cel-Col for 7 days.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Cell Culture

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Effect of l -lysine concentration on total protein content in MG63 osteoblast-like cells.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Concentration Assay

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Selected metabolites in the lysine degradation pathway. (A) The initial steps of the lysine degradation pathway and (B) the metabolite concentrations from MG63 cells cultured on PLLA, PLLA-Cel, PLLA-Col, and PLLA-Cel-Col for 7 days. The bar graph illustrating the mean ± SD ( n = 3) of each metabolite concentration (μM) is derived from 1 H NMR metabolomic data. The paired bars indicate a comparison of means; (★) and (★★), and (★★★) indicate significant differences when p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Cell Culture, Concentration Assay, Derivative Assay, Comparison

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cytotoxicity effect on MG63 ( a ) and BMSCs ( b ) after 24 h of exposure to the tested samples’ extracts, evaluated via the release of LDH enzyme. Complete growth media was used as a negative control, and lysed cells were used as a positive control. Results are expressed as mean ± standard deviation (n ≥ 3, independent samples).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Negative Control, Positive Control, Standard Deviation

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cellular viability of MG63 ( a ) and BMSCs ( b ) cultured with 100% extracts from each sample, for 1 day. Complete growth media was used as a control. The dashed line indicates the 70% cut-off for a toxic effect. Results are expressed as mean ± standard deviation (n ≥ 3, independent samples, * p < 0.05).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Cell Culture, Control, Standard Deviation

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cellular viability of MG63 ( a ) and BMSCs ( b ) cultured with 100% extracts from each sample for 3 days. Results are expressed as mean ± standard deviation (n ≥ 3; independent samples; * p < 0.05).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Cell Culture, Standard Deviation

Journal: Molecular Nutrition & Food Research

Article Title: Saucerneol Inhibits the Growth, Migration, and Invasion of Osteosarcoma Cells In Vitro and Prevents Metastasis‐Associated Osteolysis Ex Vivo

doi: 10.1002/mnfr.70187

Figure Lengend Snippet: Effects of Sauc on cell death, apoptotic cell morphology, and migration in human osteosarcoma cells. (A, B) MG63 cells (A) and SJSA‐1 cells (B) were seeded in 96‐well plates and cultured with Sauc at the indicated concentrations for 24 h. The numerical values of cytotoxicity are presented as a bar graph normalized to those of the control. (C, D) MG63 cells (C) and SJSA‐1 cells (D) were seeded in six‐well plates and cultured with Sauc at the indicated concentrations for 24 h. The morphological changes were observed using the Olympus CKX53 inverted microscope. Scale bar: 200 µm. (E, F) After 24 h of Sauc treatment of MG63 cells (E) and SJSA‐1 cells (F), cell migration was assessed using the wound healing assay. The migration rate is presented as a bar graph. Scale bar: 200 µm. All values are expressed as mean ± SD of the results from three independent experiments. * Indicates a statistically significant difference, with p < 0.05 compared to the control.

Article Snippet: Human osteosarcoma cell lines MG63 (#CRL‐1427) and SJSA‐1 (#CRL‐2098) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, Cell Culture, Control, Inverted Microscopy, Wound Healing Assay